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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/10/2008 |
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Data da última atualização: |
22/01/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; OLIVEIRA, M. C. de S.; ROCHA, R. B.; SILVA NETTO, F. G. da; CAVALCANTE, F. A. |
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Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; RODRIGO BARROS ROCHA, CPAF-RO; Francelino Goulart da Silva Netto, Embrapa Rondônia; FRANCISCO ALOISIO CAVALCANTE, CPAF-AC. |
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Título: |
Estudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1α. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. |
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Idioma: |
Português |
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Conteúdo: |
Bovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 μl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided MenosBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 μl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable regio... Mostrar Tudo |
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Palavras-Chave: |
Anaplasma marginele; Estudo genético advergência. |
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Thesagro: |
Gene; Proteína. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/116311/1/17860.pdf
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Marc: |
LEADER 02609nam a2200205 a 4500
001 1048520
005 2015-01-22
008 2008 bl uuuu u00u1 u #d
100 1 $aBRITO, L. G.
245 $aEstudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1α.
260 $aIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV$c2008
520 $aBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 μl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided
650 $aGene
650 $aProteína
653 $aAnaplasma marginele
653 $aEstudo genético advergência
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aROCHA, R. B.
700 1 $aSILVA NETTO, F. G. da
700 1 $aCAVALCANTE, F. A.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
10/03/2009 |
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Data da última atualização: |
06/06/2018 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
GAMA, E. E. G. e; PEREIRA FILHO, I. A.; CRUZ, J. C. |
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Afiliação: |
Elto Eugênio Gomes e Gama, Embrapa Milho e Sorgo; ISRAEL ALEXANDRE PEREIRA FILHO, CNPMS; JOSE CARLOS CRUZ, CNPMS. |
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Título: |
Cultivares de milho indicadas para a produção de minimilho. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: PEREIRA FILHO, I. A. (Ed.). Minimilho: cultivo e processamento. Sete Lagoas: Embrapa Milho e Sorgo, 2008. p. 21-26. |
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Idioma: |
Português |
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Palavras-Chave: |
Minimilho. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 00504naa a2200145 a 4500
001 1491829
005 2018-06-06
008 2008 bl uuuu u00u1 u #d
100 1 $aGAMA, E. E. G. e
245 $aCultivares de milho indicadas para a produção de minimilho.$h[electronic resource]
260 $c2008
653 $aMinimilho
700 1 $aPEREIRA FILHO, I. A.
700 1 $aCRUZ, J. C.
773 $tIn: PEREIRA FILHO, I. A. (Ed.). Minimilho: cultivo e processamento. Sete Lagoas: Embrapa Milho e Sorgo, 2008. p. 21-26.
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Embrapa Milho e Sorgo (CNPMS) |
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